Volume 2 Supplement 1
0099. Nitrite reductase activity during sepsis
© Simon et al; licensee Springer. 2014
Published: 26 September 2014
Nitric oxide (NO) excess is considered to be the main cause of hypotension in sepsis. Research has mainly focussed upon NO production by isoforms of NO synthase (NOS). However, alternative pathways may make an important contribution, including nitrite (NO2 -) reduction by the reductase activity possessed by numerous heme- and pterin-based enzymes. The temporal contribution of these pathways to NO production in sepsis is currently unclear.
To investigate changes in nitrite reductase activity during sepsis.
Male Wistar rats (approx 300g wt) with tunnelled right jugular venous and left common carotid arterial lines in situ received i.p. injection of either faecal slurry (septic) or n-saline (sham). Fluid (1:1 mixture of 5% glucose/Hartmann`s; 10 ml/kg/h) was started 2h later. At either 6h or 24h, rats were anaesthetized and tracheotomized. After stabilization, measurements were made of haemodynamics (blood pressure, echocardiography) and methaemoglobinaemia (oxidation of Hb to the Fe3+ form induced by NO or nitrite) before and after a 25 ml/kg bolus fluid challenge (BL, baseline) to ensure adequate LV filling. Animals then received (A) a single dose of sodium nitrite (NaNO2, 15mg/kg i.v.) to stimulate nitrite reductase activity, (B) a combination of NaNO2 and the NO-scavenger cPTIO (3.4mg/kg i.v.) or (C) a combination of NaNO2 and the non-specific NOS-inhibitor SEITU (1mg/kg i.v.). Recordings were made over the next 2-4h prior to sacrifice.
In this rat model, increased nitrite reductase activity was apparent during established (24h) sepsis, but not at an early (6h) timepoint. The clinical relevance of this finding needs to be elucidated.
Internal lab funds and salary support from University Hospital Aachen.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.