Volume 2 Supplement 1

ESICM LIVES 2014

Open Access

0854. Metabolomic changes by mass spectrometry in lung tissue from septic rats with mechanical ventilation-induced lung injury

  • Y Rojas1, 2,
  • S Naz3,
  • N Nin4, 5,
  • A Garcìa3,
  • A Ferruelo1, 2,
  • L Martínez-Caro1, 2,
  • M de Paula1, 6,
  • C Barbas3 and
  • JA Lorente1, 2, 7
Intensive Care Medicine Experimental20142(Suppl 1):P63

DOI: 10.1186/2197-425X-2-S1-P63

Published: 26 September 2014

Objective

To identify metabolomic changes in lung tissue associated with lung injury induced by mechanical ventilation (VILI) in animals with sepsis, using for the first time a global unbiased metabolomic fingerprinting approach.

Methods

Rats received cecal-ligation and puncture (CLP) or sham operation, and 24 h later underwent mechanical ventilation for 2.5 h with either VT=9 ml/kg, positive end-expiratory pressure (PEEP)=0 cm H2O (n=9 and n=12, without and with CLP, respectively); or VT=25 ml/kg, PEEP=5 cm H2O (n=13 and n=12, without and with CLP, respectively). Lung tissue samples were obtained and analyzed by nontargeted global fingerprinting approach for lung tissue analysis, applying multiple complementary analytical techniques, including liquid cromatography-mass spectrometry (MS), gas cromatography-MS, and capillary electrophoresis-MS. We followed the Principles of Laboratory Animal Care (2010/63/UE 22-09, RD 53/2013 BOE 1-02, ley 32/2007 BOE 7-11).

Results

Metabolomic changes characteristic of sepsis and VILI were identified. Lung tissue samples from septic rats with VILI were characterized by a specific metabolomic profile as compared to samples from septic rats without VILI. Metabolomic changes indicated increased oxidative stress, and changes in purine, energy, carnitine, aminoacid, urea cycle, vitamines, collagen, ceramide-sphingomyelin and phospholipid metabolism.

Conclusion

A particular metabolomic profile can be identified in lung tissue from septic rats with lung injury induced by mechanical ventilation.

Declarations

Grant acknowledgment

FIS 12/02898, FIS 11/02791, FIS 12/02451, European Network (7th FP) ITN 264864, CA11/00260.

Authors’ Affiliations

(1)
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES)
(2)
Intensive Care Service, Hospital Universitario de Getafe
(3)
Facultad de Farmacia, Centro de Metabolómica y Bioanálisis (CEMBIO), Universidad CEU San Pablo
(4)
Intensive Care Service, Hospital Español
(5)
Intensive Care Service, Hospital de Torrejon
(6)
Hospital Universitario de Getafe
(7)
Universidad Europea de Madrid

Copyright

© Rojas et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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