Volume 2 Supplement 1
0968. The influence of hypothermia and catecholamines on guinea pig's small bowel motility in vitro
© Schörghuber et al; licensee Springer. 2014
Published: 26 September 2014
In critically ill patients early enteral nutrition (EN) preserves gastrointestinal (GI) integrity and motility and should be started as early as possible. We know that several therapeutic strategies, e.g. catecholamines or analgosedation, exert adverse effects on GI motility.1 What we do not know is whether therapeutic hypothermia has an influence on GI motility and thereby feeding intolerance.
The aim of this study was to find out if guinea pig's small bowel motility is altered during hypothermia and after rewarming and if catecholamines cause alterations of peristalsis in this situation.
Guinea pig´s small bowel segments of 8 cm length were set up in organ baths containing oxygenated Tyrode´s solution. Peristalsis was elicited by luminal perfusion (0.5 ml/min) against an aboral resistance of 400 Pascal (Pa). Perfusion of the segments resulted in an increase of the intraluminal pressure up to a pressure threshold (PT; mean ±SEM), where peristaltic contractions were triggered. The pressure was recorded at the aboral end of the segments. An increase of the PT indicates an inhibition of peristalsis, while a decrease of the PT represents a stimulation of peristalsis. A PT of 400 Pa was equated with a complete block of peristalsis. PT was firstly measured at 37°C temperature of the organ bath, after rapid cooling to 20°C and after rewarming to 37°C (control). In a second setting before rewarming one of the following substances were added to the organ baths: adrenaline 100 nM, dobutamine 100 µM, noradrenaline 1 µM. At 37°C PT was evaluated again.
PT and start of peristalsis after admission of catecho/amines compared to control. 1two-sided t-test. 2Kruskal-Wallis.
Start of peristalsis after rewarming (%)
PT (Pa) after rewarming
70.4 ± 8.3
182.7 ± 39.3
264.2 ± 46.6
223.9 ± 48.4
Our experimental setting demonstrates a distinct impairment of small bowel motility during hypothermia, a delayed restart and a persistent inhibition of motility in the presence of catecholamines, explaining the higher incidence of feeding intolerance in this group of patients.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.