Plasma volumes. Plasma volumes at baseline (PV1), at 3 h after the surgical preparation just before the start of treatment (PV2) and at the end of the experiment (PV3). There was no significant difference between any of the groups at any time points. There was a significant difference between PV1 and PV2, and PV2 and PV3 for all groups. Two-way ANOVA for repeated measures followed by Bonferroni post hoc test was used for the statistical analyses (**p < 0.01).