Gene expression of lung inflammatory mediators obtained by real-time PCR in lung homogenates. Lung samples for gene expression analysis were obtained at BD (at the end of donor management), after 8 h of cold storage (Ischemia), at the end of reperfusion follow-up (Graft) and compared to controls (Control). Cytokines, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF α), and interferon gamma (IFN γ) are shown in (A); chemokines, chemokine C-C motif ligand 2 (CCL2-MCP-1), chemokine CXC motif ligand 10 (CXCL-10), interleukin-8 (IL-8), and the oxidative stress index high mobility group box-1 (HMGB-1) are shown in (B); endothelial mediators: endothelin-1 (EDN-1), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and selectin-E (SELE) are shown in (C). The error bars show the standard deviation of the mean. One-way ANOVA vs. Control. P < 0.05 accepted as significant: asterisk (*) vs. Control.