Bronchoalveolar lavage fluid (BALF) concentration of (A) IL-6 and (B) MIP-2, from wild-type (WT, white boxes), and Tpl2-deficient mice (Tpl2-/-, gray boxes) subjected only to sample collection (control, n=5-8), mechanical ventilation with normal tidal volume for 240 min, or high tidal volume for 210 min (n=5-7), and WT mice subjected to 210 min of high tidal volume ventilation treated with a Tpl2 inhibitor, either prior (Tpl2inh pre, n=5), or after initiation of high VT ventilation (Tpl2inh post, n=5). BALF IL-6 was lower in genotype-matched control mice than in ventilated mice, *p<0.05. High tidal volume-induced increase in BALF IL-6 was greater in WT untreated mice, than in Tpl2-/- mice, and WT mice treated with the Tpl2 inhibitor, *p<0.05. BALF MIP-2 concentration was higher in WT mice ventilated with high VT than in WT control mice *p<0.001. BALF MIP-2 concentration was also higher in WT mice ventilated with high VT than in similarly ventilated Tpl2-/- mice, and WT mice pre-treated with the Tpl2 inhibitor, *p<0.001.