Figure 3
From: Acute organ injury is associated with alterations in the cell-free plasma transcriptome
Representative fluorescently labeled cDNA prepared using the Affymetrix small-sample method. It is run on an Agilent 2100 Bioanalyzer both with and without previous template switching amplifications. Amplification results in a greater area under the curve, representing the mass of incorporated cDNA (2 μg), are compared to only 40 ng in an unamplified sample. The peak also shifts right with amplification, indicating larger cDNA sequences.