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Volume 3 Supplement 1

ESICM LIVES 2015

Sepsis results in early cholesterol and steroidogenesis pathway alterations

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Introduction

Both steroid and sex hormones regulate multiple physiological functions including the stress response, fluid retention, metabolism and reproduction. They are synthesized from cholesterol via the precursor steroid pregnenolone. We previously reported early falls in HDL and LDL cholesterol levels in a fluid-resuscitated rat model of faecal peritonitis [1] and these could prognosticate as early as 6 h post-insult [2]. Pregnenolone may be converted to either (i) progesterone, corticosterone and then aldosterone via a common pathway or (ii) to dehydroepiandrosterone (DHEA), the precursor to testosterone and estradiol.

Objectives

Using our 72 h rat model of faecal peritonitis, we sought to investigate the relationship between hypocholesterolaemia and abnormalities in the steroidogenesis pathways in sepsis.

Methods

Awake, instrumented yet fully mobile male Wistar rats (325 ± 15g) received an i.p. injection of 4µl/g faecal slurry. Fluid resuscitation (50:50 mix of 5% glucose/Hartmann's; 10 ml/kg/h) was commenced at 2 h. At 6 h, an echo-measured heart rate cut-off of 460 bpm was used to classify animals into predicted survivors or non-survivors. Blood samples were taken concurrently for measurement of the lipid profile (enzymatic colorimetric analysis) and plasma levels of progresterone, corticosterone, aldosterone, DHEA, testosterone and estradiol (ELISA). Control animals were treated identically except for slurry injection. Results were analysed using two-way ANOVA and post-hoc testing and considered statistically significant when p < 0.05.

Results

At 6 h septic animals had significant hypocholesterolaemia, the magnitude of which was significantly greater in predicted non-survivors. Levels in the progesterone-corticosterone-aldosterone steroidogenesis pathway were all elevated (p < 0.05 vs controls), whereas DHEA-testosterone-estradiol steroidogenesis pathway levels were all subnormal (Table 1).

Table 1 Table 1

Conclusions

Sepsis resulted in significant early reductions in cholesterol levels. This reduction may be due to increased cholesterol utilization for preferential steroidogenesis in the progesterone-corticosterone-aldosterone pathway, at the expense of DHEA-testosterone-estradiol steroidogenesis. This relationship has not, to our knowledge, been previously described in sepsis and warrants further investigation.

Grant Acknowledgment

ESICM Basic Science Award, Intensive Care Foundation (UK), NIHR

References

  1. 1.

    Khaliq W, et al: Intensive Care Med Experimental. 2014, 2 (S1): 22-

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Author information

Correspondence to W Khaliq.

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Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Khaliq, W., Andreis, D. & Singer, M. Sepsis results in early cholesterol and steroidogenesis pathway alterations. ICMx 3, A978 (2015) doi:10.1186/2197-425X-3-S1-A978

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Keywords

  • Cholesterol
  • Testosterone
  • Estradiol
  • Aldosterone
  • Dehydroepiandrosterone