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Figure 3 | Intensive Care Medicine Experimental

Figure 3

From: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)

Figure 3

NET-related markers are released by neutrophils in a dose- and time-dependent fashion. The kinetics of B. pseudomallei-induced NET formation at various multiplicities of infections (MOI; 10°, 101, 102) and time points (0, 1, and 4 h) were assessed by quantifying elastase (A, C) and extracellular DNA using a Picogreen dsDNA kit (B, D), in the supernatant of stimulated isolated human neutrophils. The negative control (−) was medium (HBSS−/−), and the positive control (+) was PMA, a known inducer of NETs. Both elastase and DNA increase in a time- and dose-dependent manner following B. pseudomallei (Bps) stimulation. The mean and SD are expressed. (E) The two NET markers, elastase and DNA, were highly correlated, suggesting that neutrophils are the source of this extracellular DNA. All experiments used whole blood from three to six different healthy human volunteers. Elastase results were log-transformed to correct for heteroscedasticity. P values reported are for the slope of linear regression models with elastase or DNA as the dependent variables and time point or MOI as independent variables. **P < 0.01; ***P < 0.001.

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