Figure 5

Evaluation of TNF, IL-6, and NF-κB expressions in the kidney. Upper figures: The evaluation of tumor necrosis factor (TNF)-α expression in the vascular endothelial cells of the kidney. TNF-α protein was stained in brown, and the level of TNF-α expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of the TNF-α expression scores in the left kidney (LK) and right kidney (RK). ANP, atrial natriuretic peptide; IRI, ischemia-reperfusion injury. Middle figures: The evaluation of interleukin (IL)-6 expression in the vascular endothelial cells and proximal convoluted tubules of the kidney. IL-6 protein was stained in brown, and the level of IL-6 expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of the IL-6 expression scores in the left kidney (LK) and right kidney (RK). Lower figures: The evaluation of nuclear factor (NF)-κB expression in the proximal convoluted tubules of the kidney. NF-κB protein was stained in brown, and the level of NF-κB expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of the NF-κB expression scores in the left kidney (LK) and right kidney (RK).