Figure 6

Evaluation of TNF, IL-6, and NF-κB expressions in the lung. Upper figures: The evaluation of tumor necrosis factor (TNF)-α expression in the bronchial epithelial cells of the lung. TNF-α protein was stained in brown, and the level of TNF-α expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of the TNF-α expression scores in the lung. ANP, atrial natriuretic peptide; IRI, ischemia-reperfusion injury. Middle figures: The evaluation of IL-6 in the columnar epithelial cells of the bronchioles of the lung. IL-6 protein was stained in brown, and the level of IL-6 expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of IL-6 expression score in the lung. Lower figures: The evaluation of nuclear factor (NF)-κB expression in the bronchioles of the lung. NF-κB protein was stained in brown, and the level of NF-κB expression was scored: score 0 (hardly stained), score 1 (weakly stained), score 2 (moderately stained), score 3 (strongly stained). Comparison of NF-κB expression score in the lung.