Cells with DNA damage in hippocampus and basal cortex. Bar graphs demonstrate mean TUNEL-positive cell count of both hemispheres (±SD) subdivided into controls (n = 5) and different degrees of CPP depletion (mild, n = 5; moderate, n = 3; severe, n = 4). Quantification was performed by counting TUNEL-positive cells co-localizing with DAPI (blue) in regions of interest in the hippocampus (A) and basal cortex (B). Histochemistry (C): Co-localization with DAPI (left column) confirmed that TUNEL-positive staining (middle column) was generally in the nucleus (right column). Hollow arrows show DAPI-positive nuclear staining (blue). Filled arrows identify TUNEL-positive cells. Scale bars = 50 μm. *p < 0.05. **p < 0.01.