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Intensive Care Medicine Experimental
Figure 2 | Intensive Care Medicine Experimental

Figure 2

From: Shape descriptors of the “never resting” microglia in three different acute brain injury models in mice

Figure 2

Shape descriptor and grid crossing protocols. Shape descriptors (area, perimeter, circularity, Feret’s diameter, aspect ratio, and solidity) were quantified on 179 × 133 μm fields (A). The original image was scaled to microns and segmented by applying the Max function on Fiji (A’, see methods). Objects with > 25 μm2 area were selected and analyzed (A’’, outlines of selected objects for analysis). For the grid crossing counts, a grid with 9 × 9 μm spaced lines was used (B). This was overimposed to the image (B’) and intersections between > 25 μm2 objects (previously segmented), and the grid were quantified (B’’). Scale bar = 20 μm.

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