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Figure 3 | Intensive Care Medicine Experimental

Figure 3

From: Shape descriptors of the “never resting” microglia in three different acute brain injury models in mice

Figure 3

GFP+ microglia distribution over the lesion area at 24 h after tMCAo, pMCAo, or TBI. GFP+ microglia showed homogenous distribution over the lesioned hemisphere after tMCAo (A). Microglial hypertrophic morphology was evident both in the cortex (B) and the striatum (lesion core, C). On the contralateral side GFP+ cells displayed the typical ramified morphology with thin branches (D). Within the lesion core, GFP (green) co-localized with CD11b (red, pan-marker of microglia/macrophages, E) only in ramified CD11b+ cells. No co-localization was observed between GFP and round-shaped CD11b+ (E) or CD45high+ cells (infiltrating leukocytes, F). After pMCAo (G) GFP+ microglia were not present in the lesion core (H). Hypertrophic GFP+ cells surrounded the lesion and placed at lesion border (I). Normal ramified morphology was observed in contra-lateral side (J). The lesion core was populated by round-shaped GFP-/CD11b+ and GFP- /CD45high+ cells (K, L). After TBI (M) GFP+ microglia had similar appearance as to pMCAo: absent in the lesion core (N), hypertrophic at lesion border (O) and ramified with thin branches in contra-lateral side (P). The lesion core was populated by round-shaped GFP-/CD11b+ and GFP- /CD45high+ cells (Q, R). Data are representative of 3 independent experiments. Scale bars= 20 μm.

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