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Fig. 6 | Intensive Care Medicine Experimental

Fig. 6

From: Atelectasis causes alveolar hypoxia-induced inflammation during uneven mechanical ventilation in rats

Fig. 6

Effect of hypoxia on cytokine secretion from MLE15 and MH-S cells and activities of NF-κB and HIF-1α in MLE15 cells. a, b Cells were cultured in 21 % O2 (white bar) or 5 % O2 (black bar) with or without TNF-α (500 pg/mL). a CXCL-1 secreted from MLE15 cells and b CCL-2 secreted from MH-S cells. (n = 6 samples per group). c–f MLE15 cells were cultured in 21 % O2 (white bar) or 5 % O2 (black bar) with TNF-α (500 pg/mL). c NF-κB p65 binding activities (n = 5 samples per group) and d HIF-1α concentrations in MLE15 cells (n = 4 samples per group). e VEGFA and f GLUT1 mRNA levels in MLE15 cells (n = 5 samples per group). *p < 0.05 compared with the 21 % O2 group, †p < 0.05 compared with the group without TNF-α. TNF-α tumor necrosis factor α, CXCL-1 chemokine (C-X-C motif) ligand 1, CCL-2 chemokine (C-C motif) ligand 2, NF-κB nuclear factor-κB, HIF-1 hypoxia-inducible factor 1, VEGFA vascular endothelial growth factor A, GLUT1 glucose transporter-1

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