Fig. 7
Effects of NF-κB inhibition and HIF-1α knockdown on cytokine secretion from MLE15 cells. Cells were cultured in 21 % O2 (white bar) or 5 % O2 (black bar) with TNF-α (500 pg/mL). a CXCL-1 secretion from MLE15 cells cultured with 500 pg/mL TNF-α after treatment with Bay 11–7082 or vehicle. *p < 0.05 compared with the 21 % O2 group (n = 6 samples per group). b HIF1A, VEGFA, and GLUT1 mRNA levels and c HIF-1α protein concentrations in MLE15 cells transfected with HIF-1α or nontarget siRNA. *p < 0.05 compared with the nontarget groups (n = 5 samples per group). d CXCL-1 secretion from MLE15 cells cultured with 500 pg/mL TNF-α after transfection with HIF-1α or nontarget siRNA. *p < 0.05 compared with the 21 % O2 group, †p < 0.05 compared with the nontarget group (n = 6 samples per group). NF-κB nuclear factor-κB, HIF-1 hypoxia-inducible factor 1, VEGFA vascular endothelial growth factor A, GLUT1 glucose transporter-1, K/D knockdown, CXCL-1 chemokine (C-X-C motif) ligand 1