Table 1 Primers for qPCR
Gene | Primer | Sequence |
|---|---|---|
Mouse HIF1A | Forward | 5′-ATCAAGTCAGCAACGTGGAA-3′ |
Reverse | 5′-AATGGGTTCACAAATCAGCAC-3′ | |
Rat and mouse VEGFA | Forward | 5′-TGCTGTACCTCCACCATGC-3′ |
Reverse | 5′-GATGTCCACCAGGGTCTCAA-3′ | |
Rat GLUT1 | Forward | 5′-CCCTGCAGTTCGGCTATAA-3′ |
Reverse | 5′-AGTGTGGTGAGTGTGGTGGA-3′ | |
Mouse GLUT1 | Forward | 5′-TTATTGCCCAGGTGTTTGG-3′ |
Reverse | 5′-GTTACGATTGATGAGCAGGAAG-3′ | |
Rat ACTB | Forward | 5′-TGACGTTGACATCCGTAAAGAC-3′ |
Reverse | 5′-AGAGCCACCAATCCACACA-3′ | |
Mouse ACTB | Forward | 5′-TGACAGGATGCAGAAGGAGA-3′ |
Reverse | 5′-GCTGGAAGGTGGACAGTGAG-3′ |