Fig. 2

NF-κB activity in Drosophila melanogaster after septic injury with Staphylococcus aureus. a We determined NF-κB expression at 6, 18, 48, and 168 h (1 week) after needle pricking with qRT-PCR. Expression of dif and Dorsal 18 h after infection was upregulated in infected with antibiotics group and was significantly higher compared to that of sham; expression of Relish was upregulated in all three groups with significant difference; all three NF-κB components (dorsal, Dif, Relish) remained elevated by 1 week following survival from sepsis (*p < 0.05). b Luciferase activity in live flies expressing NF-κB luciferase reporter was measured for 72 h after infection. Flies demonstrated elevated levels of NF-κB activation in both infected without treatment and infected with antibiotics groups in the first 24 h and sustained elevation of NF-κB activity up to 72 h in infected with antibiotics flies, while activity in sham group was not upregulated after pricking (*p < 0.05)