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Intensive Care Medicine Experimental
Fig. 2 | Intensive Care Medicine Experimental

Fig. 2

From: Modulation of monocytes in septic patients: preserved phagocytic activity, increased ROS and NO generation, and decreased production of inflammatory cytokines

Fig. 2

Strategy to detect intracellular cytokine (IL-6) in monocytes and CD163+ and CD163− subsets. a Individual cells (singlets) were selected, and monocytes were characterized by light scatter and side positivity for CD14. Dot plots were drawn with IL-6 versus the side scatter parameter. The quadrants for IL-6 positivity were established based on unstimulated cells (control), and the percentages of positive stained cells were determined after stimulation. b CD163+ and CD163− subsets of monocytes were established based on isotype control, and the percentages of cells positively stained for IL-6 were measured after stimulation. The graphics are representative of an experiment for IL-6 detection in a healthy volunteer and in a septic patient in unstimulated cells and after stimulation with P. aeruginosa

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