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Intensive Care Medicine Experimental
Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Modulation of monocytes in septic patients: preserved phagocytic activity, increased ROS and NO generation, and decreased production of inflammatory cytokines

Fig. 3

Detection of ROS, NO, IL-6, and TNF-α in monocytes of healthy volunteers and patients with severe sepsis and septic shock. Whole blood was obtained from 19 healthy volunteers (blank boxes), 19 patients with severe sepsis (boxes in light gray), and 15 patients with septic shock (boxes in dark gray). Monocyte analysis was performed by assessing individual cells (singlets) combined with the side-scatter parameter versus CD14 positiveness. The values for ROS and NO are shown as geometric mean fluorescence intensities (GMFI) of DCFH and DAF, respectively. Cytokine values are shown as percentages of cells producing IL-6 and TNF-α. P < 0.001 for all stimuli compared between groups (Kruskal-Wallis). *P < 0.05 compared to healthy volunteers, # P < 0.05 compared to severe sepsis patients (Mann-Whitney)

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