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Fig. 4 | Intensive Care Medicine Experimental

Fig. 4

From: Modulation of monocytes in septic patients: preserved phagocytic activity, increased ROS and NO generation, and decreased production of inflammatory cytokines

Fig. 4

Interaction of CD163 expression on monocytes with the production of ROS, NO, IL-6, and TNF-α. Whole blood of 34 septic patients (D0) (solid line) and 19 healthy volunteers (dot line) were analyzed after stimulation with LPS, P. aeruginosa, and S. aureus. Values represent the means for each variable in CD163+ and CD163− monocytes. Monocyte analysis was performed by assessing individual cells (singlets) combined with side-scatter parameters versus CD14 positiveness. Monocytes were further characterized as CD163+ and CD163− cells. The productions of ROS and NO were analyzed in histogram charts and quantified as the geometric mean fluorescence intensities (GMFIs) associated with the detection of DCFH and DAF, respectively. Intracellular cytokine level was based on quadrants established in the sample without stimulation and are expressed as the percentage of cytokine-producing monocytes. *P values denote the interaction between CD163 expression and the groups of healthy volunteers and septic patients for each parameter and condition evaluated (ANOVA)

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