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Intensive Care Medicine Experimental
Fig. 6 | Intensive Care Medicine Experimental

Fig. 6

From: Modulation of monocytes in septic patients: preserved phagocytic activity, increased ROS and NO generation, and decreased production of inflammatory cytokines

Fig. 6

Co-localization of gp91phox (red) and p47phox (green) by immunofluorescence. PBMCs were obtained from septic patients at admission (D0; n = 20) and after 7 days of follow-up (D7; n = 10) and from healthy subjects (n = 10). a. The cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, blue) and gp91phox and p47phox using specific antibodies. The images were obtained using a confocal microscope (objective ×60; ×5 amplification). b. The graph represents the mean fluorescence intensity (MFI) obtained by co-localization analysis (colocalizationhighlighter) using ImageJ software (National Institutes of Health, Bethesda, MD, USA). *P < 0.05

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