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Intensive Care Medicine Experimental
Fig. 4 | Intensive Care Medicine Experimental

Fig. 4

From: Hypercapnic acidosis attenuates pulmonary epithelial stretch-induced injury via inhibition of the canonical NF-κB pathway

Fig. 4

Effects of HCA on NF-κB pathway mediated via IκBα. ELISA with embedded Western blot demonstrating that HCA prevents the stretch-induced decrement in cytoplasmic concentrations of the NF-κB inhibitor IκBα (a). Direct overexpression of IκBα attenuates the deleterious effects of stretch in normocapnia-exposed epithelial layers, decreasing NF-κB activation (b), reducing epithelial LDH leakage (c) and preserving cell viability (d). HCA did further reduce medium LDH but had no effect on NF-κB or on cell viability, in the presence of prior IκBα overexpression. Note: *P < 0.05 versus normocapnia-empty vector, † P < 0.05 versus normocapnia-IκBα-SR. Abbreviations: Normocapnia-Sham unstretched cells with normocapnia, Normocapnia-Stretch high cyclical stretch with normocapnia, HCA-Sham unstretched cells with normocapnia, HCA-Stretch high cyclical stretch with hypercapnia

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