Fig. 6
From: Sepsis modeling in mice: ligation length is a major severity factor in cecal ligation and puncture
Clinical and biological characteristics of the 20 % ligated group. Results are reported as median ± IQR. a Invasive mean arterial pressures (MAP) were measured in the 20 % ligated and sham groups before surgery and at 24 h post-procedure. At 24 h after surgery, the animals presented a decreased MAP below 65 mmHg compared to sham mice. *p < 0.05. b TNF-α, IL-1β, IL-6, IL-10, and IL-6/IL-10 ratio serum concentrations were measured at 6 h (H6), 24 h (H24), and 48 h (H48) after surgery. We expressed serum cytokine concentrations as fold increases relative to time-matched sham laparotomy (n = 10 for the sham-operated and 20 % group at H6, H24, and H48). We observed a significant imbalance in favor of pro-inflammatory status. § p < 0.05 for IL-1β between H6 and H24, and H6 and H48 after CLP. # p < 0.05 for TNF-α between H6 and H24 and H6 and H48 after CLP. &p < 0.05 for IL-6/IL-10 between H24 and H48. c, d Leukocyte (c), lymphocyte (d), and neutrophil (e) counts in the 20 % ligated group before surgery, at H6, H24, and H48. We observed a drop after surgery, more pronounced at H24, with an increase at 48 h. *p < 0.05