Fig. 3
From: Heparin-binding protein is important for vascular leak in sepsis
Effects of enzymatic removal of heparan sulfate or chondroitin sulfate by Heparinase III and Chondroitinase ABC on HBP-induced permeability increases. EA.hy926 cells were grown to confluence on permeable supports and treated with heparinase III (Hep. III) or Chondroitinase ABC (Chondro. ABC) for 1 h and then stimulated with HBP. a TEER was measured after 1.5 h after HBP stimulation. b HRP was also added to the top chamber, and HRP passage was measured 2 h after HBP stimulation. TEER values are normalized to the TEER of empty inserts. Error bars are standard error of the mean, n = 3 for each condition. In both experiments, one-way ANOVA with Dunnett’s test for multiple comparisons was used to compare each group to the condition with HBP and no enzyme treatment (far right). *P < 0.05, **P < 0.01, ***P < 0.001