Fig. 4
From: Heparin-binding protein is important for vascular leak in sepsis
Effect of inhibition of signaling pathways in HBP-induced permeability increases. EA.hy926 cells were grown to confluence on permeable supports and treated with Y-27 632 (Rho-kinase inhibitor) and Calphostin C (PKC inhibitor) for 1 h and then stimulated with HBP. TEER across the filter was measured 1.5 h after HBP stimulation and is normalized to empty inserts. Error bars are standard error of the mean, n = 3 for each condition. One-way ANOVA with Dunnett’s test for multiple comparisons was used to compare each group to the condition with HBP and no inhibitor (far right). *P < 0.05, **P < 0.01, ***P < 0.001