Fig. 8
From: Heparin-binding protein is important for vascular leak in sepsis
Unfractionated heparin reversed HBP-induced permeability increases. EA.hy926 cells were grown to confluence on permeable supports and stimulated with 10 μg/mL HBP. At 1 h following stimulation, HBP-containing media were removed and replaced with fresh media (gray line, treatment 1), or heparin was added to a final concentration of 3 U/mL (dashed line, treatment 2), or no change was made (black line, treatment 3). TEER across the filter was monitored over time and is normalized to empty inserts. The overall difference was determined by two-way repeated measures ANOVA (treatment effect P = 0.002, time effect P < 0.001). Sidak’s multiple comparisons post hoc test was used to compare treatments 1 and 2 to treatment 3 at each time point. Error bars are standard error of the mean, n = 3 for each intervention. UFH unfractionated heparin. **P < 0.01, ***P < 0.00