Effects of N-acetylcysteine (NAC) supplementation in resuscitation fluids on renal microcirculatory oxygenation, inflammation, and function in a rat model of endotoxemia

Ergin, Bulent; Guerci, Philippe; Zafrani, Lara; Nocken, Frank; Kandil, Asli; Gurel-Gurevin, Ebru; Demirci-Tansel, Cihan; Ince, Can

doi:10.1186/s40635-016-0106-1

Intensive Care Medicine Experimental

Table 3 Time-course of acid-base status, lactate levels, and hematocrit resuscitated with and without the addition of NAC

From: Effects of N-acetylcysteine (NAC) supplementation in resuscitation fluids on renal microcirculatory oxygenation, inflammation, and function in a rat model of endotoxemia

	T0 (baseline)	T1 (shock)	T2 (30 min)	T3 (120 min)
pH
Time control	7.37 ± 0.03	7.39 ± 0.06	7.42 ± 0.03	7.42 ± 0.04
Control + NAC	7.48 ± 0.12	7.42 ± 0.03	7.38 ± 0.02	7.32 ± 0.05*
LPS	7.36 ± 0.03	7.27 ± 0.06**	7.26 ± 0.07***	7.2 ± 0.07***
LPS + HES-RA	7.36 ± 0.03	7.26 ± 0.07***	7.31 ± 0.07*	7.27 ± 0.05***
LPS + HES-RA + NAC	7.37 ± 0.04	7.26 ± 0.09***	7.27 ± 0.08***	7.20 ± 0.03***
HCO₃ ⁻ (mmol/L)
Time control	20.6 ± 0.9	21 ± 0.6	21.2 ± 1.3	21.6 ± 1.3
Control + NAC	18.8 ± 0.6	18.4 ± 1.5*	19.3 ± 1.2	14.2 ± 3.8***
LPS	20.2 ± 0.7	15 ± 0.9***	15.3 ± 1.2***	12.8 ± 0.5***
LPS + HES-RA	21.6 ± 0.7	14.8 ± 1.5***	18.1 ± 0.9***^, ⁺⁺	16.7 ± 1.6***, ⁺⁺⁺
LPS + HES-RA + NAC	20.7 ± 0.8	14.7 ± 2.5***	16.6 ± 1.6***	14 ± 1.7***^{, ##}
Base excess (mmol/L)
Time control	−3.5 ± 1.2	−2.7 ± 1.6	−1.7 ± 1.3	−1.8 ± 1.5
Control + NAC	−3.5 ± 0.5	−4.4 ± 1.7	−4.5 ± 1.3	−8.9 ± 2.4***
LPS	−4 ± 1.3	−10.8 ± 1.9***	−10.5 ± 1.7***	−14.1 ± 1.3***
LPS + HES-RA	−2.9 ± 1.1	−11.3 ± 2.7***	−7 ± 2***^{, ++}	−8.9 ± 1.9***^{, +++}
LPS + HES-RA + NAC	−3.4 ± 1.5	−11.2 ± 4.1***	−9.1 ± 2.8***	−12.5 ± 1.7***^{, ##}
Anion gap K⁺ (mmol/L)
Time control	17.7 ± 0.8	18.0 ± 0.7	16.4 ± 0.5	16.8 ± 1.3
Control + NAC	18.9 ± 2.0	18.5 ± 2.0	17.0 ± 1.2	18.9 ± 4.8
LPS	18.9 ± 2.3	21.8 ± 1.1	21.6 ± 1.8	23.2 ± 1.6***
LPS + HES-RA	17.5 ± 1.3	22.7 ± 1.3	19.2 ± 1.0	20.9 ± 2.5***
LPS + HES-RA + NAC	18.4 ± 1.0	22.5 ± 2.5**	20.6 ± 2.3*	23.1 ± 1.9***
Lactate (mmol/L)
Time control	2.32 ± 0.52	2.22 ± 0.34	2.08 ± 0.31	1.73 ± 0.27
Control + NAC	3.10 ± 0.64	2.92 ± 0.76	2.17 ± 0.36	2.10 ± 0.33
LPS	2.42 ± 0.32	3.08 ± 0.28	3.08 ± 0.5	4.32 ± 0.6***
LPS + HES-RA	1.92 ± 0.2	3.32 ± 0.55	2.75 ± 0.48	5.08 ± 1.83***
LPS + HES-RA + NAC	2.70 ± 0.21	3.83 ± 1.36**	3.30 ± 1.17 ^*	4.98 ± 1.21***
Hct (%)
Time control	49.6 ± 1.3	41.6 ± 1.9	39 ± 2.9	33.3 ± 3
Control + NAC	48.8 ± 2.6	42.8 ± 4.4	41.1 ± 4.2	33.8 ± 2.6
LPS	49.3 ± 4.0	39.6 ± 3.0	35.1 ± 2.9	34.0 ± 3.5
LPS + HES-RA	49.3 ± 1.7	43.3 ± 2.5	30.1 ± 4.5***^{, +}	17.5 ± 3***^{, +++}
LPS + HES-RA + NAC	48.6 ± 0.8	44.1 ± 3.2 ⁺	33.0 ± 2.6**	18.0 ± 2.0***^{, +++}

LPS lipopolysaccharide, HES-RA hydroxyethyl starch-ringer acetate, NAC N-acetylcysteine
Values are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 versus control; ⁺⁺ p < 0.05, ⁺⁺⁺ p < 0.001 versus LPS; ^## p < 0.01 versus LPS + HES-RA

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