Fig. 4

Reduced lung injury in HDC^−/− mice following CLP-induced sepsis. Lung tissues were harvested from sham-operated and CLP-induced septic mice 24 h after surgery. a Lung sections stained with hematoxylin and eosin. Original magnification, ×200. A bar graph shows semiquantitative analysis of lung tissues by lung injury score, which was performed by scoring from 0–4 as described in Methods. A total lung injury score was calculated by adding the individual scores in every animal and averaging the total values in each group (n = 4–10/group). b Sections were stained with antibody against MPO followed by peroxidase staining. Original magnification, x200 or x400. A bar graph shows the summary of quantitation of MPO-positive cell counts. The average of MPO-positive cell number in three fields per sample was calculated (n = 4–10/group). c Wet-to-day ratios of lungs harvested from the animals were determined to assess pulmonary edema (n = 6/group). The summarized results are presented as means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the respective control (24 h after sham operation). ^## P < 0.01 vs. CLP WT