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Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy

Fig. 3

Nlrp3 KO mice have a survival benefit and do not lose body or muscle weight during 96 h of sepsis. Twelve- to 16-week-old male Nlrp3 KO and WT mice were subjected to CLP or sham surgery. a Survival curves show an improved survival of Nlrp3 KO compared to WT mice following CLP surgery. All sham mice survived to the experimental end point. Survival analysis was performed with Log-Rank-test; WT sham vs. WT CLP: p ≤ 0.001, (circle); Nlrp3 KO sham vs. Nlrp3 KO CLP: p ≤ 0.05, (number sign); WT CLP vs. Nlrp3 KO CLP: p ≤ 0.05 (section sign). The numbers of animals in each experimental group are indicated in the figure. b Body weight at 96 h after surgery. CLP-treated Nlrp3 KO (n = 16); sham Nlrp3 KO (n = 8), WT CLP (n = 12), WT sham (n = 13). c, d qRT-PCR analysis of Il6 expression in gastrocnemius/plantaris and tibialis anterior muscles of WT sham (n = 5), WT CLP (n = 9), Nlrp3 KO sham (n = 5), and Nlrp3 KO CLP (n = 6) mice. mRNA expression was normalized to Gapdh. e, f Weights of the skeletal muscles, e gastrocnemius/plantaris (GP), and f tibialis anterior (TA) determined at 96 h after surgery. CLP treated Nlrp3 KO (n = 16); sham Nlrp3 KO (n = 8), WT CLP (n = 12), WT sham (n = 13). All weights were normalized to tibia length and expressed as percent-wise change compared to the respective sham group. Data are presented as the mean ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ns = not significant

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