Fig. 4

Nlrp3 KO mice are protected from inflammation-induced muscle atrophy. 12–16-week-old male Nlrp3 KO and WT mice were subjected to CLP or sham surgery. a H&E and ATPase stain of histological cross sections from gastrocnemius/plantaris (GP) and tibialis anterior (TA) muscle from sham and CLP-operated WT and Nlrp3 KO mice at 96 h after surgery, as indicated. Scale bar = 100 μm. b Quantification of fast/type II myofiber cross-sectional area (MCSA) in GP and TA from transverse sections stained with metachromatic ATPase dye assay (as shown in a). MCSA was determined by using ImageJ software. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001