Fig. 5

Disturbed muscular protein homeostasis in sepsis is blunted in Nlrp3 KO mice. Twelve- to 16-week-old male Nlrp3 KO and WT mice were subjected to CLP or sham surgery. a Western blot analysis with anti-myosin heavy chain (MyHC) slow and anti-MyHC fast antibody and anti-MuRF1 antibody GAPDH was used as loading control; n = 3. b qRT-PCR analysis of myosin heavy chain (Myh) 2, Myh4, and Myh7 expression in gastrocnemius/plantaris (GP) muscles of sham and CLP mice at 96 h after surgery as indicated (WT sham: n = 5; WT CLP: n = 9; Nlrp3 KO sham n = 5; Nlrp3 KO: CLP n = 6). mRNA expression was normalized to Gapdh. (c–f) qRT-PCR analysis of Trim63 (MuRF1) and Fbxo32 (atrogin 1) expression in gastrocnemius/plantaris and tibialis anterior muscle of sham and CLP-operated WT and Nlrp3 KO mice at 96 h after surgery as indicated (WT sham: n = 5; WT CLP: n = 9; Nlrp3 KO sham n = 5; Nlrp3 KO: CLP n = 6). mRNA expression was normalized to Gapdh. Data are presented as mean ± SEM. ns = not significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001