Fig. 3

Leukocyte recruitment in the hepatic microcirculation in response to resuscitation with colloid solutions of albumin. Intravital microscopy was used to measure leukocyte rolling (a) and adhesion (b) in hepatic post-sinusoidal venules, as well as sinusoidal leukocyte adhesion (c) and blood flow (d) in CLP or sham mice at 6 h after surgery. All mice were resuscitated with either 5 mL/kg of normal saline containing 5% albumin, or novel Seplyte L or H fluids containing 5% albumin, in accordance with clinical dosing practice. Fluids were administered intravenously immediately following surgery and 4 h later. Data are presented as mean ± SEM with n = 5–6 for each group. *p < 0.05, **p < 0.01, ***p < 0.001; asterisk directly above error bar indicates comparison to sham control. CLP cecal ligation and puncture, NS normal saline, SepL Seplyte L, SepH Seplyte H, 5% Alb 5% albumin