Fig. 5

Adipogenic potential of patient serum of lean and overweight/obese prolonged critically ill patients. hADSCs were supplemented with serum from either healthy volunteers (n = 47), lean prolonged critically ill patients (BMI ≤25 kg/m², n = 20), or overweight/obese prolonged critically ill patients (BMI ≤25 kg/m², n = 20). a Proliferation presented as the number of cells per square millimeter. b Oil Red O staining presented as the number of stained pixels per cells, expressed as a fold of the mean of the healthy conditions per day. c Relative mRNA expression of CEBPB. d Relative mRNA expression of DLK1. Gene expression data are expressed normalized to GAPDH and as a fold change of the mean of the day 0 condition. Light gray bar or light gray line mean of conditions with serum from healthy controls, white bar or black line serum from lean patients, dark gray bar or dotted black line serum from overweight/obese patients [*p ≤ 0.05 compared to healthy condition, ^§ p ≤ 0.05 compared to previous day, ⁺ p = 0.09 compared to healthy condition, ^x p ≤ 0.05 between lean and overweight/obese conditions, ^£ p ≤ 0.05 between lean and healthy conditions]