Fig. 2

Granulocyte and monocyte adsorptive apheresis (GMA) selectively adsorbed activated neutrophils and monocytes of peripheral blood in septic rats. a White blood cells were counted at 0, 1, and 2 h during the GMA treatment; neutrophil and monocyte numbers were determined from stained cytospins by the Wright-Giemsa staining (n = 5–6 per group). b Scanning electron microscopy images of the cellulose acetate beads after GMA treatment. c Leukocytes were adsorbed onto the cellulose acetate beads after GMA treatment and were stained with DAPI (blue) and CD11b (red) at ×200 ×Z 1.0 magnification. d, e Peripheral blood neutrophils were collected at 0 and 2 h during GMA treatment. The mean fluorescence intensity of CD11b was analyzed by flow cytometry (n = 5–6 per group). *p < 0.05, **p < 0.01, ***p < 0.001. Data are expressed as mean ± SEM. WBC white blood cell, DAPI 4′,6-diamidino-2-phenylindole