Fig. 3

Superoxide dismutase 2 (SOD2) immunocontent and gene expression after sepsis induction by CLP. Three times, 3 h (a), 6 h (b) and 12 h (c), after sepsis, the renal arteries were isolated, and the content of SOD2 was quantified by immunoblot. Representative western blots show samples from shams in the first bands and samples from sepsis in the last bands, represented in equal number. The densitometric analysis quantification is depicted in bar graphs. Gene expression of SOD2 was measured by RT-PCR, with b-actin as control, 6 h (d) and 12 h (e) after the induction of sepsis in sham and sepsis groups. The results are expressed as optical densitometry for the mean ± S.E.M. (n = 6 animals in each group). *p < 0.05 compared to the sham group