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Intensive Care Medicine Experimental
Fig. 5 | Intensive Care Medicine Experimental

Fig. 5

From: Extracellular superoxide dismutase is necessary to maintain renal blood flow during sepsis development

Fig. 5

Levels of 3-nitrotyrosine by ELISA and iNOS expression by blotting after sepsis induction by CLP. At times 3 h (a), 6 h (b) and 12 h (c) after sepsis induction, the renal arteries were isolated, and the content of 3-nitrotyrosine was quantified by ELISA and expressed in terms of nitrotyrosine concentration/absorbance. The content of iNOS was measured by immunoblot 3 h (d), 6 h (e) and 12 h (f) after sepsis. Representative western blots showed samples from shams in the first bands and samples from sepsis in the last bands, represented in equal number. The densitometric analysis quantification is depicted in bar graphs. The results are expressed as the mean ± S.E.M. in arbitrary units. (n = 6 animals in each group). *p < 0.05 compared to the sham group

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