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Intensive Care Medicine Experimental

Table 2 Nucleotide sequences of the primers used in the q-PCR experiments

From: The effects of hyperoxia on microvascular endothelial cell proliferation and production of vaso-active substances

Target mRNA

Target protein

Forward primer

Reverse primer

NOSIII

eNOS

TGGCTTTCCCTTCCAGTTC

AGAGGCGTTTTGCTCCTTC

EDN1

ET-1

TGTGTCTACTTCTGCCACCTG

TGGCTAGCACATTGGCATCTA

ACTB

Beta-actin

AACTCCATCATGAAGTGTGACG

GATCCACATCTGCTGGAAGG

  1. NOSIII/eNOS endothelial nitric oxide synthase, EDN1 preproendothelin-1, ET-1 endothelin-1
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