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Table 1 Score sheet to assess sepsis-induced behaviour changes

From: Improving animal welfare using continuous nalbuphine infusion in a long-term rat model of sepsis

Rat grimace scale

Clinical scoring*

Orbital tightening

0□

1□

2□

Reduced activity

0□

1□

2□

Nose/cheek flattening

0□

1□

2□

Sunken flanks

0□

1□

2□

Ear changes

0□

1□

2□

Back arching

0□

1□

2□

Whisker changes

0□

1□

2□

Piloerection

0□

1□

2□

    

Bloated abdomen

0□

1□

2□

    

Chromodacryorrhea (= eye discharge)

0□

1□

2□

  1. Legend: The score sheet was adapted from [17] and www.ahwla.org.uk. *Six or more points in the clinical scoring lead to a preterm abortion of the experiment by injection of 1 ml phenobarbital i.v.