Fig. 1

Schematic diagram illustrating the experimental protocol. After surgery, the sheep recovered for 60 min. Renal ischemia was caused by clamping of the left renal artery for 90 min. Fifteen minutes prior to the release of the clamp, intravenous infusions of either the organic mononitrites of 1,2-propanediol (PDNO) or vehicle (1,2-propanediol + inorganic nitrite) were commenced. The infusions continued for 6 h, during which renal function was monitored. The arrows indicate times for measurements and blood-/urine-sampling performed at baseline, prior to treatment, prior and directly following the release of the clamp, and continuously every hour after treatment was started