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Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Intra-cellular lactate concentration in T lymphocytes from septic shock patients — a pilot study

Fig. 3

Correlation between intracellular and supernatant lactate concentration in healthy volunteer’s T lymphocytes. T lymphocytes were purified from six healthy donors’ blood. Cells were cultured in complete RPMI medium at a concentration of one million cells/mL, and stimulated with 4 μg/mL PHA (black circles) or left unstimulated (NS, open circles). After the previously determined 40-h incubation period, supernatant and one million T cells were aliquoted and lactate concentration was measured in supernatants and in T cells. Results are expressed as the quantity of lactate per well of the lecture plate, which contains 50 μL of the obtained cell lysate or supernatant. Each point represents the mean of the duplicates for each donor. Dotted line represents the linear regression model and grey zone represents the 95% confidence interval of the regression. Correlation between intra-lymphocyte and supernatant lactate concentration was explored with Pearson correlation

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