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Fig. 4 | Intensive Care Medicine Experimental

Fig. 4

From: Intra-cellular lactate concentration in T lymphocytes from septic shock patients — a pilot study

Fig. 4

Minimal cell number for lactate measurement in healthy volunteers’ T lymphocytes. T lymphocytes were purified from four healthy donors’ blood. Cells were cultured in complete RPMI medium at a concentration of one million cells/mL and were stimulated with 4 μg/mL PHA (black circles) or left unstimulated (NS, open circles). After the previously determined 40-h incubation period, different number of T cells (ranging from 100,000 to 1,000,000) were aliquoted and intra-lymphocyte lactate concentrations measured. Results are expressed as the quantity of lactate per well of the lecture plate, which contains 50 μL of the obtained cell lysate. Each point represents the mean of the duplicates for each donor and horizontal bars represent the median value

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