Alterations in mitochondrial respiration and reactive oxygen species in patients poisoned with carbon monoxide treated with hyperbaric oxygen

Intensive Care Medicine Experimental

Table 2 Mitochondrial respiration and H₂O₂ production

	Control group		CO group (pre-HBO)		CO group (post-HBO)
Respiratory states	Mean	SEM	Mean	SEM	Mean	SEM
Mitochondrial respiration (measured in pmol O₂ × s^− 1 × 10^− 6 PBMCs)
Routine	19.56	0.54	13.23	1.42	22.10	1.28
LEAK	13.33	0.97	6.19	0.99	13.54	1.81
OXPHOS	20.57	0.87	8.47	1.45	17.51	2.58
ETS	35.66	2.20	18.39	2.42	35.23	3.70
ETS-CI	41.73	2.76	19.81	1.77	38.07	3.61
ETS-CI&II	58.04	2.33	32.27	3.17	60.54	3.32
ETS-CII	20.67	2.11	16.44	1.30	28.07	2.25
Gp	55.86	5.96	47.86	6.36	47.15	3.88
ROX	1.17	0.20	0.73	0.19	0.76	0.20
CIV	63.60	6.03	43.52	2.92	76.50	4.30
Mitochondrial H₂O₂ production (measured in pmol H₂O₂ × s⁻¹ × 10⁻⁶ PBMCs)
Routine	0.06	0.02	0.04	0.02	0.16	0.07
LEAK	0.18	0.05	0.24	0.13	0.92	0.14
OXPHOS	0.25	0.05	0.16	0.05	0.93	0.22
ETS	0.22	0.04	0.14	0.02	0.85	0.20
ETS-CI	0.22	0.04	0.13	0.02	0.66	0.21
ETS-CI&II	0.22	0.04	0.17	0.02	1.14	0.32
ETS-CII	0.26	0.06	0.23	0.04	0.71	0.08
ROX	0.57	0.15	0.31	0.06	1.59	0.42

Cellular mitochondrial respiration and hydrogen peroxide production obtained in permeabilized PBMCs between the control group, CO (pre-HBO) group, and the same CO group after undergoing HBO treatment (post-HBO). Values presented as mean ± SEM