Fig. 3

Modified Murine Sepsis Score (MSS) over time (a), MSS score as a predictor of death (b), and MSS individual component scores (c–f) over time in mice subjected to moderate or severe CLP. Data are presented as mean ± SEM from six independent experiments representing a total number of n = 18 sham, n = 23 moderate CLP, and n = 17 severe CLP mice; *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham (or vs. line of identity in (b)); ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. moderate CLP, by two-way ANOVA. Note: Only two mice undergoing severe CLP survived past 18 h, therefore statistical comparisons were not performed at this time-point (a, c–f). Dotted line in (b) represents the line of identity for statistical comparison in ROC analyses. Level of consciousness, activity, and response to stimuli scores were the same for each mouse, and therefore are displayed together in (d). AUC area under the curve, 95% CI 95% confidence interval