Fig. 4

Modified Mouse Clinical Assessment Score for Sepsis (M-CASS) over time (a), M-CASS score as a predictor of death in septic mice (b), and individual M-CASS component scores (c–f) over time in mice undergoing moderate or severe CLP. Data are presented as mean ± SEM from six independent experiments representing a total number of n = 18 sham, n = 23 moderate CLP, and n = 17 severe CLP mice; *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham (or vs. line of identity in (b)); ^###p < 0.001 vs. moderate CLP, by two-way ANOVA. Note: Only two mice undergoing severe CLP survived past 18 h, therefore statistical comparisons were not performed at this time-point (a, c–e). Dotted line in (b) represents the line of identity for statistical comparison in ROC analyses. Fur Aspect, Activity, and Eyelid component scores are identical to scores in Fig. 3c–e, respectively, thus are not shown again here, but are included as part of the overall M-CASS score. AUC area under the curve, 95% CI 95% confidence interval