Fig. 5
a Relation between liver uncoupling protein 2 (UCP 2) mRNA gene expression and liver mitochondrial H2O2 production. UCP 2 mRNA was determined by real-time quantitative PCR in the liver of control groups (naïve and sham-operated) (white symbols) white symbols) and CLP groups (black symbols). Data are means ± SEM. *p < 0.05 versus sham-operated and naïve rats; &p < 0.05 compared 24 h CLP and 36 h CLP, from five animals per group determined by surgical procedure and following period (0, 6, 24, 36 h). Mitochondrial H2O2 production was measured in the presence of succinate without rotenone. *p < 0.05 versus sham-operated and naïve rats; &p < 0.05 compared 24 h CLP and 36 h CLP, from six animals per group determined by surgical procedure and following period (0, 6, 24, 36 h). b Mitochondrial transcription factor A (mTFA) and nuclear respiratory factor 1 (NRF 1) mRNA level were determined by real-time quantitative PCR in the liver of control groups (naïve and sham-operated) (white symbols) and CLP groups (black symbols). Data are means ± SEM. *p < 0.05 versus sham-operated and naïve rats and †p < 0.05 compared 6 h CLP and 36 h CLP, from five animals per group determined by surgical procedure and following period (0, 6, 24, 36 h)