Group | CG n = 9 | SG n = 9 | HG n = 9 | HSG n = 9 |
|---|
Leucocytes (× 103/mL) | 97.07 ± 13.66 | 93.72 ± 10.48 | 64.99 ± 10.48 a,b | 71.65 ± 13.66a,b |
Macrophages (× 103/mL) | 94.23 ± 13.33 | 87.86 ± 13.33 | 48.35 ± 6.39 a,b | 66.96 ± 11.06c |
Lymphocytes (× 103/mL) | 2.28 ± 1.06 | 1.63 ± 2.04 | 2.48 ± 1.20 | 2.16 ± 2.20 |
Neutrophils (× 103/mL) | 0.56 ± 0.54 | 4.23 ± 3.14 | 14.16 ± 11.45 a,b | 2.53 ± 2.18c |
- aControl group (CG)—control mouse exposed to normoxia in air room, without surfactant. Surfactant group (SG)—mice were challenged with exogenous surfactant (surfactant challenge 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia). Hyperoxia group (HG)—the animals were exposed to 100% oxygen in chamber for 24 h. For more details, see the “Methods” section. Hyperoxia-surfactant group (HSG)—mice were challenged with exogenous surfactant (surfactant challenge - 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia) after exposure lungs to 100% oxygen in chamber for 24 h. significant difference between the groups when compared to the CG; bsignificant difference between the groups when compared to the SG; csignificant difference between the groups when compared to the HG. Data are expressed as mean ± SD and were analyzed by one-way ANOVA followed by Newman-Keuls’s post-test (p < 0.05)
