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Table 2 Biochemical analysis of damage and oxidative stress and inflammatory cytokines in lung tissue homogenates

From: Exogenous surfactant prevents hyperoxia-induced lung injury in adult mice

Group

CG n = 9

SG n = 9

HG n = 9

HSG n = 9

SOD (U/mg ptn)

84.25 ± 7.68

76.26 ± 8.70

59.47 ± 5.25 a

93.63 ± 23.43c

CAT (U/mg ptn)

1.45 ± 0.46

1.09 ± 0.24

1.26 ± 0.18

1.57 ± 0.39

TBARS (nmol/mg ptn)

1.63 ± 0.15

1.72 ± 0.15

2.04 ± 0.24a,b

1.47 ± 0.44c

Protein carbonyl (nmol/mg ptn)

18.15 ± 1.91

15.58 ± 1.97

25.34 ± 1.86a,b

18.01 ± 5.08c

TNFα (pg/mL)

154.5 ± 8.21

156.6 ± 10.98

226.0 ± 33.96 a,b

152.5 ± 34.47c

CCL5 (pg/mL)

351.5 ± 68.68

345.4 ± 58.46

259.2 ± 45.63 a,b

221.9 ± 61.08 a,c

IL-17 (pg/mL)

351.9 ± 45.78

352.6 ± 148.70

616.0 ± 84.07 a,b

333.2 ± 77.31 c

  1. Control group (CG)—control mouse exposed to normoxia in air room, without surfactant. Surfactant group (SG)—mice were challenged with exogenous surfactant (surfactant challenge 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia). Hyperoxia group (HG)—the animals were exposed to 100% oxygen in chamber for 24 h. For more details, see the “Methods” section. Hyperoxia-surfactant group (HSG)—mice were challenged with exogenous surfactant (surfactant challenge 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia) after exposure lungs to 100% oxygen in chamber for 24 h. SOD superoxide dismutase, CAT catalase, TBARS thiobarbituric acid reactive substances, TNF tumor necrosis factor, CCL5 C-C motif chemokine ligand 5 (RANTES), IL-17 Interleukin-17. asignificant difference between the groups when compared to the CG; bsignificant difference between the groups when compared to the SG; csignificant difference between the groups when compared to the HG. Data are expressed as mean ± SD and were analyzed by one-way ANOVA followed by Newman-Keuls’s post-test (p < 0.05)