Exogenous surfactant prevents hyperoxia-induced lung injury in adult mice

Intensive Care Medicine Experimental

Table 3 Hematologic parameters in BALB/c mice after hyperoxia and/or exogenous surfactant

Group	CG n = 9	SG n = 9	HG n = 9	HSG n = 9
Erythrocyte (× 10⁶/mm³)	7.70 ± 0.65	7.80 ± 0.60	9.09 ± 0.30^a,b	8.13 ± 0.42^c
Hematrocit (%)	37.08 ± 3.89	37.68 ± 2.66	44.34 ± 1.19 ^a,b	39.90 ± 1.66^c
Hemoglobin (g/dL)	12.36 ± 1.56	12.56 ± 0.91	14.78 ± 0.59 ^a,b	13.30 ± 1.66^c
Leucocytes (× 10³/mL)	4.07 ± 0.93	4.05 ± 0.53	2.55 ± 0.48 ^a,b	3.04 ± 0.60
Monocyte (×10³/mL)	0.85 ± 0.40	0.75 ± 0.18	0.37 ± 0.08 ^a	0.73 ± 0.19
Lymphocytes (× 10³/mL)	2.55 ± 0.85	3.08 ± 0.73	2.12 ± 0.37	2.26 ± 0.43
Neutrophils (× 10³/mL)	0.67 ± 0.27	0.22 ± 0.17 ^a	0.06 ± 0.02 ^a	0.05 ± 0.01 ^a

Control group (CG)—control mouse exposed to normoxia in air room, without surfactant. Surfactant group (SG)—mice were challenged with exogenous surfactant (surfactant challenge 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia). Hyperoxia group (HG)—the animals were exposed to 100% oxygen in chamber for 24 h. For more details, see the “Methods” section. Hyperoxia-surfactant group (HSG)—mice were challenged with exogenous surfactant (surfactant challenge 2.0 mL/kg/day) by intranasal instillation (12 h before hyperoxia) after exposure lungs to 100% oxygen in chamber for 24 h. ^asignificant difference between the groups when compared to the CG; ^bsignificant difference between the groups when compared to the SG; ^csignificant difference between the groups when compared to the HG. Data are expressed as mean ± SD and were analyzed by one-way ANOVA followed by Newman-Keuls’s post-test (p < 0.05)