Fig. 1

Schematic representation of the factors which influence cell biodistribution in vivo. The source of MSCs (mainly adipose, cord or marrow), culture conditions, pre-activation strategies and the method of storage before administration influences the quantity and quality of MSCs and conditioned media (MSC-CM) produced. The decision to use either the MSCs themselves or MSC-CM, as well as considering the condition to be treated, will influence the chosen route, dosage and timing of administration which will in turn influence the biodistribution pattern of the cells or cell products. Biodistribution data will furnish knowledge of the safety, efficacy, viability, behaviour and clearance rate which will feedback to the optimal source, culture, storage, route, dosage and timing strategies